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customized primers containing both illumina adapters and distinct barcode sequences  (Illumina Inc)


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    Structured Review

    Illumina Inc customized primers containing both illumina adapters and distinct barcode sequences
    Customized Primers Containing Both Illumina Adapters And Distinct Barcode Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/customized+primers+containing+both+illumina+adapters+and+distinct+barcode+sequences/customized+primers+containing+both+illumina+adapters+and+distinct+barcode+sequences/pm37910306-43-14-11
    Average 90 stars, based on 1 article reviews
    customized primers containing both illumina adapters and distinct barcode sequences - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Assessments of Bacterial Community Shifts in Sediments along the Headwaters of São Francisco River, Brazil
    Article Snippet: .. PCR amplifications were performed in triplicate using customized primers containing both Illumina adapters and distinct barcode sequences so that each amplified DNA sample contained a different combination of barcodes in order to distinguish the libraries after the sequencing step [35]. .. All PCR reactions were carried out using the optimized PCR reaction mix OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer (New England Biolabs), with the following thermocycling PCR program: 94 ◦C for 3 min; 25 cycles of 94 ◦C for 45 s, 50 ◦C for 60 s, 72 ◦C for 90 s, and 72 ◦C for 10 min.

    Article Title: Spatial distribution of sediment bacterial communities from São Francisco River headwaters is influenced by human land-use activities and seasonal climate shifts.
    Article Snippet: Riverbed sediments are dynamic freshwater environments colonized by a great diversity of microorganisms which play important roles in supporting freshwater ecosystem by performing a vast array of metabolic functions.. Recent evidence generated by HTS approaches has revealed that the structure of sediment microbial communities is influenced by natural seasonal variations in water such as temperature or streamflow as well by disturbances caused by local human activities.. Here, a spatiotemporal analysis of sediment microbial distribution from São Francisco River headwaters section was conducted using Illumina 16S rRNA-V4 region amplicon sequencing in order to accomplish three major goals: (i) to investigate whether the diversity and composition of bacterial communities accessed in riverbed sediments vary in response to distinct land-use activities; (ii) to estimate whether the diversity patterns vary between the dry and wet seasons; and (iii) to evaluate whether the diversity of bacterial metabolic functions, predicted by PICRUSt2 approach, varies similarly to the estimated taxonomic diversity.

    Amplification:

    Article Title: Assessments of Bacterial Community Shifts in Sediments along the Headwaters of São Francisco River, Brazil
    Article Snippet: .. PCR amplifications were performed in triplicate using customized primers containing both Illumina adapters and distinct barcode sequences so that each amplified DNA sample contained a different combination of barcodes in order to distinguish the libraries after the sequencing step [35]. .. All PCR reactions were carried out using the optimized PCR reaction mix OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer (New England Biolabs), with the following thermocycling PCR program: 94 ◦C for 3 min; 25 cycles of 94 ◦C for 45 s, 50 ◦C for 60 s, 72 ◦C for 90 s, and 72 ◦C for 10 min.

    Article Title: Spatial distribution of sediment bacterial communities from São Francisco River headwaters is influenced by human land-use activities and seasonal climate shifts.
    Article Snippet: Riverbed sediments are dynamic freshwater environments colonized by a great diversity of microorganisms which play important roles in supporting freshwater ecosystem by performing a vast array of metabolic functions.. Recent evidence generated by HTS approaches has revealed that the structure of sediment microbial communities is influenced by natural seasonal variations in water such as temperature or streamflow as well by disturbances caused by local human activities.. Here, a spatiotemporal analysis of sediment microbial distribution from São Francisco River headwaters section was conducted using Illumina 16S rRNA-V4 region amplicon sequencing in order to accomplish three major goals: (i) to investigate whether the diversity and composition of bacterial communities accessed in riverbed sediments vary in response to distinct land-use activities; (ii) to estimate whether the diversity patterns vary between the dry and wet seasons; and (iii) to evaluate whether the diversity of bacterial metabolic functions, predicted by PICRUSt2 approach, varies similarly to the estimated taxonomic diversity.

    Sequencing:

    Article Title: Assessments of Bacterial Community Shifts in Sediments along the Headwaters of São Francisco River, Brazil
    Article Snippet: .. PCR amplifications were performed in triplicate using customized primers containing both Illumina adapters and distinct barcode sequences so that each amplified DNA sample contained a different combination of barcodes in order to distinguish the libraries after the sequencing step [35]. .. All PCR reactions were carried out using the optimized PCR reaction mix OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer (New England Biolabs), with the following thermocycling PCR program: 94 ◦C for 3 min; 25 cycles of 94 ◦C for 45 s, 50 ◦C for 60 s, 72 ◦C for 90 s, and 72 ◦C for 10 min.

    Article Title: Spatial distribution of sediment bacterial communities from São Francisco River headwaters is influenced by human land-use activities and seasonal climate shifts.
    Article Snippet: Riverbed sediments are dynamic freshwater environments colonized by a great diversity of microorganisms which play important roles in supporting freshwater ecosystem by performing a vast array of metabolic functions.. Recent evidence generated by HTS approaches has revealed that the structure of sediment microbial communities is influenced by natural seasonal variations in water such as temperature or streamflow as well by disturbances caused by local human activities.. Here, a spatiotemporal analysis of sediment microbial distribution from São Francisco River headwaters section was conducted using Illumina 16S rRNA-V4 region amplicon sequencing in order to accomplish three major goals: (i) to investigate whether the diversity and composition of bacterial communities accessed in riverbed sediments vary in response to distinct land-use activities; (ii) to estimate whether the diversity patterns vary between the dry and wet seasons; and (iii) to evaluate whether the diversity of bacterial metabolic functions, predicted by PICRUSt2 approach, varies similarly to the estimated taxonomic diversity.



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    Illumina Inc customized primers containing both illumina adapters and distinct barcode sequences
    Customized Primers Containing Both Illumina Adapters And Distinct Barcode Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/customized+primers+containing+both+illumina+adapters+and+distinct+barcode+sequences/customized+primers+containing+both+illumina+adapters+and+distinct+barcode+sequences/pm37910306-43-14-11
    Average 90 stars, based on 1 article reviews
    customized primers containing both illumina adapters and distinct barcode sequences - by Bioz Stars, 2026-11
    90/100 stars
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